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Effects of different nutrient media and explant types on in vitro shoot regeneration of medical species of Origanum minutiflorum O. Schwarz & Davis Dudu Ozkum, R.Tipirdamaz.

Yazar: Materyal türü: MakaleMakaleDil: İngilizce Yayın ayrıntıları:2007. Internatıonal Socıety Hortıcultural Scıence,ISSN:
  • 0567-7572
Konu(lar): LOC sınıflandırması:
  • T49
İçindekiler: Proceedings of the IIIrd Balkan Symposium on Vegetable and Potatoes 2007, Issue 729, p129-132 Özet: In vitro shoot regeneration procedure for oregano explants (O. minutiflorum O. Schwarz & Davis), endemic medical species of Turkey, is described. Hypocotyl, shoot apex, leaf disc and single nodal segment explants excised from 15 d old in vitro germinated seedlings were cultured on Murashige and Skoog (MS) and B5 media supplemented with combination of 1.5 mg L-1 benzylaminopurine (BAP) and 0.2 mg L naphthalene acetic acid (NAA) containing 3% sucrose and 0.6% agar. Single nodal segment explants are the best source for the highest shoot induction and development (70%) obtained on MS medium in term of shoot regeneration and no success was obtained for other explants types. However for all explants, this phyto hormone combination and 135 medium had no effective success and therefore was not suitable on in vitro shoot regeneration.
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Online Electronic Document NEU Grand Library Online electronic T49 .E34 2007 (Rafa gözat(Aşağıda açılır)) Ödünç verilmez EOL-226

In vitro shoot regeneration procedure for oregano explants (O. minutiflorum O. Schwarz & Davis), endemic medical species of Turkey, is described. Hypocotyl, shoot apex, leaf disc and single nodal segment explants excised from 15 d old in vitro germinated seedlings were cultured on Murashige and Skoog (MS) and B5 media supplemented with combination of 1.5 mg L-1 benzylaminopurine (BAP) and 0.2 mg L naphthalene acetic acid (NAA) containing 3% sucrose and 0.6% agar. Single nodal segment explants are the best source for the highest shoot induction and development (70%) obtained on MS medium in term of shoot regeneration and no success was obtained for other explants types. However for all explants, this phyto hormone combination and 135 medium had no effective success and therefore was not suitable on in vitro shoot regeneration.

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