| 000 | naa a22 7ar4500 | ||
|---|---|---|---|
| 016 | _a000307981500002 | ||
| 022 | _a1572-3887 | ||
| 040 | _aNEU | ||
| 041 | _aeng | ||
| 050 | _aQU4.3 | ||
| 100 | 1 |
_9574912 _aDalmızrak, Özlem, _cProf. Dr. _ |
|
| 245 | 1 | 0 |
_aEvaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi. _cOzlem Dalmizrak, Gulnihal Kulaksiz, Erkmen Nazmi Ozer. |
| 260 |
_c2012. _bSpringer, _aNew York : |
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| 520 | _aSt John's Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. The principal of the study was to clarify the effects of hypericin, one of the components of SJW, on glutathione S-transferase-pi (GST-pi) purified from human placenta. The K (m) values of GST-pi were 0.21 +/- A 0.03 mM for glutathione (GSH) and 2.29 +/- A 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the V (m) value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 +/- A 30 U/mg protein versus 74 +/- A 3 U/mg protein. At constant substrate concentrations (1 mM), an average IC (50) value of 0.70 +/- A 0.02 mu M was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a K (i) value of 0.31 +/- A 0.05 mu M was found; when CDNB was the varied substrate, a K (i) value of 0.85 +/- A 0.02 mu M was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi. | ||
| 650 | 0 |
_9117600 _aBiochemistry |
|
| 650 | 0 |
_9572720 _aNear East University Article |
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| 650 | 0 |
_9572723 _aYakın Doğu Üniversitesi Makale |
|
| 650 | 0 |
_9576675 _aInhibition kinetics |
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| 650 | 0 |
_9576676 _aSt John's Wort |
|
| 650 | 0 |
_9576677 _aTransplacental transfer |
|
| 773 |
_aProtein Journal _gOCT 2012, Vol 31 Issue 7, p544-549 _x15723887 |
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| 856 | _uhttp://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1007/s10930-012-9433-6 | ||
| 942 |
_x1000007 _kQU00000043E932012 _cOED |
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| 005 | 20160223114342.0 | ||
| 008 | 150427t xxu||||| |||| 00| 0 tu d | ||
| 001 | 267712 | ||
| 999 | _c244376 | ||